مطالعه اثر انواع مختلف عسل بر رشد سویه استاندارد سودوموناس آئروژینوزا در شرایط برون‌تنی

Study of the Effects of Different Kinds of Honeys on the Growth of Standard Strain of Pseudomonas aeroginosa (in vitro) M. Saffari PhD , M. Taghizadeh PhD , M. Pourbabaee BSc Received: 01/01/06 Sending for Revision: 24/06/06 Receiving Revised Manuscript:06/09/06 Accepted: 19/09/06 Background and Objective: Pseudomonas aeroginosa is a gram negative bacterium which is found in the intestinal tract and skin as normal flora. It can cause opportunistic infections, contaminated wounds especially burn lesions, and is resistant to many antibiotics. Many studies support the use of honey for these infections. The aim of this study was to investigate the possible bactericidal effects of different kinds of honey, on P. aeroginosa, in Kashan . Materials and Methods: This was a labaratory study. Eight out of 15 honey samples were selected and their reducing sugars, saccharose were quantified, also Diastase enzyme activity was determined. The included specimens were then classified to natural (6 specimens), synthetic unnatural (2 specimens) and a sample prepared by sugar, and two by heat-inactivated natural honeys. Different concentration (V/V) containing 5, 10, 15, 20, 25, 30 percent of honeys were added in the Muller Hinton agar and P. aeroginosa (ATCC=27853) were then cultured. Results: The results showed that natural honeys at the concentration of 10%, had bactricidal effects on the growth of P.aeroginosa. The synthetic honeys did not show this effect. but heating process reduced bactricidal properties of the honeys. Conclusion: We concluded that natural honeys can be used for the treatment of infectious wounds especially in burns, and superficial lesion. In vivo studies are also warranted to confirm the bactericidal effects of honey. Key words: Diastase, Wounds, Burns, Pseudomonas aeroginosa, Honeys